Anatomy and Physiology of the Human Body

Anatomy and Physiology of the Human Body Anatomy and Physiology Part A Identify the parts of the digestive system on the diagram overleaf?(Refer to Brief) Explain the function of each part of the digestive system identified in the diagram above? Outline the composition of Proteins, Fats and Carbohydrates, and explain how each of them are digested and absorbed by the body? Part B Draw a diagram of a typical cell and state the function of each of its organelles? Classify tissues into the four main groups; epithelial, connective, muscle, and nervous, give an example of each? (Table format will suffice) Explain the difference between benign and malignant tumours? Part C Label the urinary system using the diagram overleaf?(Refer to Brief) Explain the structure and function of each element of the urinary system? Draw the structure of a Nephron and explain how it produces urine? Name and explain three diseases /disorders which affect the urinary system? Part A (ii)Explain the function of each part of the digestive system identified in the diagram above? Mouth Oesophagus Stomach Liver Gallbladder Pancreas Large Intestine Small Intestine Appendix Rectum Anus In this section I will explain each of the stages of digestion where the food travels from the mouth into each section of the digestive system until it leaves the body from the anus. Mouth This is the first part of the digestive system. The mouth is made up of the teeth, tongue and salivary glands. We put food in our mouth and chew it with our teeth the are four main types of teeth the first being incisors which we have four of in the top and bottom jaws these are sharp and cut through our food. Then we have the canines we have two in each jaw and are used for tearing our food. We then have the premolars which have four in each jaw for crushing and breaking down our food. Then we have the molars which there are six to each jaw and have the same action as the premolars. Our tongue moves the food around our mouth to our different teeth. It mixes with our saliva which is secreted from the salivary glands which contains water, mucus and the enzyme salivary amylase and when mixed with the food creates bolus which we then swallow using the aid of our tongue. (Class notes 2015) Oesophagus This is an involuntary muscular tube that leads from the mouth to the stomach. It carries the food to the stomach by muscle contraction called peristalsis. This muscle contracts and relaxes to create a wave like motion for the food to travel down smoothly also releasing mucus to lubricate the travelling of the bolus to the stomach. (Class notes 2015) Stomach Food gets to the stomach through the oesophagus and passes through the cardiac sphincter this blocks the food from travelling back up the oesophagus. In the stomach which resembles a large sac that can hold anything between 2- 4 litres of food depending on the person. The first part of digestion starts here the stomach churns the bolus around and adds enzymes to aid in the breakdown of the bolus to create chime. The stomach also releases hydrochloric acid to kill the bacteria that travel to the stomach contained in the food.(Class notes 2015) Liver The livers function in the digestive system is as follows it secretes bile into the small intestine and this is generally to breakdown the fat that has travelled to the small intestine it also takes the nutrients that have been adsorbed by the small intestine and changes them into chemicals that the body needs. It also breaks down drugs and alcohol that have been consumed. . (clevelandclinic.org 2015) Gallbladder The Gallbladder is attached to the liver and stores the bile from the liver which is used to digest and break down the fats in the small intestine this takes place in the duodenum. Pancreas The pancreas is a gland that is just behind the stomach its function is to secrete both Exocrine and Endocrine. Exocrine is the pancreatic juice that contains digestive enzymes. Endocrine contains important hormones which include insulin and glucagon. Both of these help balance the amount of sugar in the body in different ways. Large Intestine The large intestine is approx. 1.5 metres long and connects the small intestine to the rectum and anus this is draped over the small intestine its main function is to draw the last of the nutrients and water from the food passing through it the left overs are faeces and the large intestine gets rid of this waste from the body through the anus. Small Intestine The small intestine is normally about 6 meters long and approximately 90% of digestion takes place here through the main 3 parts that are known as duodenum, jejunum and ileum. On the inside walls of the small intestine are villi that work for nutrient absorption and also have a group of lymph and blood vessels. Appendix The appendix is commonly classed as a useless organ which is attached to the large intestine ascending it can store bad bacteria and this in turn can cause inflammation thus leading to appendicitis and removal of the appendix. Although it is disputed that the appendix can store good bacteria and after a bout of diarrheal illnesses it can reboot the digestive system. (webmd.com 2015) Rectum The rectum is the last part of the large intestine the length being around 12cm long and is a store house for faeces. This is the leftover food, bacteria and undigested materials such as roughage that is found in vegetables and is all stored here until the rectum walls expand and we get the urge to defecate. (healthline.com 2015) Anus This is the very last part of the digestive system we defecate through the anus and is a voluntary movement in most people but not in infants. This is where we dispel waste which contains bacteria, undigested food. Part A (iii)Outline the composition of Proteins, Fats and Carbohydrates, and explain how each of them are digested and absorbed by the body? In this section I will create a chart covering the sources, functions and digestion of proteins, fats and carbohydrates in the human body and will show there different effects and how they fuel the body with energy. Sources Function Digestion Proteins Groundnuts, beans, whole cereals, fish, pulses,meat,eggs, Milk and cheese. Protein builds the Body and repairs muscle. It’s broken down in the digestive system and travels to the muscles as amino acids. Stomach, Pepsin breaks protein into large polypeptides. Small intestine, enzymes break large polypeptides into smaller polypeptide chains. Lastly still in the intestine enzymes the small polypeptides are broken into amino acids for absorption. Fats Dairy products, meat, fish, olive oil, cake, chocolate, avocados and sunflower oil. Good fats help maintain a healthy diet and are essential to health. Bad fats cause weight gain and health problems such as clogged arteries. Small intestine Broken down by bile salts from the liver and turned into liquid. Small intestine, its broken down further into fatty acids and glycerol to be absorbed. Carbohydrates Potatoes, pasta, apples, bread, meat, fish and dairy products. Provide the body with energy for the muscles, nervous system and also help the body burn fat. Carbohydrates get broken down to monosaccharide’s to get absorbed and then will become glucose to supply the body with energy. Part B Draw the typical diagram of a cell, and state the function of each of its organelles? In this section I will draw a typical animal cell as I see it and will give a breakdown of each of its organelles and how they function in the typical cell. Cell membrane, this is the outer skin of the cell that holds everything inside the cell and keeps things outside the cell and also that controls movement into or out of the cell. Cytoplasm, this is a gel like fluid which stores nutrients and water for the cell and also helps protect the cell acting like a cushion for cell movement. Nucleus, this contains DNA and all the cells genetic characteristics and also direct the activity of the cell. Nuclear membrane, this is the layer that holds the nucleus separate from the cytoplasm. Endoplasmic reticulum, there are two types of endoplasmic reticulum these are rough (moves protein made by ribosomes) and smooth(steroid and lipid distribution) but both move the materials around the cell. Ribosomes, these are responsible for protein production in the cell and are known as the protein factories of the cell. The protein is required for cell repair and growth. Golgi apparatus, These are known as the postal system of the cell they transport package and deliver proteins lipids and enzymes throughout the cells of the body.(Class notes 2015) Mitochondria, Also known as the power house of the cell because they supply energy to the cell. Chemical reactions in the mitochondria are the difference with the cell surviving also the energy released results in the formation of ATP (adenosine triphosphate) which is the primary energy transporter in the cell. Lysosomes, clean the cell of waste generated through parts of the cell being bad and also clean the cell of bacteria. They also aid in the breakdown of food particles and then can be used for energy in the cell. Vacuoles, these are storage areas in the cell that contain secretions or waste that are made by the cytoplasm and in different types of cells are used for digestion or storage. Nucleolus, this is a tiny body inside the nucleus that directs the formation of ribosomes in the cell which then are stored in the cytoplasm of the cell. Part B (ii)Classify tissues into the four main groups; epithelial, connective, muscle, and nervous, give an example of each? (Table format will suffice) Here I will construct a table to outline the four main groups of tissues and give an example of each as I understand them. Epithelial Connective Muscle Nervous Skin Intestines Internal organs Glands Bone Cartilage Adipose Blood Skeletal muscle (voluntary) Smooth (involuntary) Cardiac (involuntary Brain Spinal cord Nerves Example The outer body is covered in epithelial skin this helps protect against infection keeping germs out and all of our blood and muscle tissue on the inside. Outer skin on the body. This connects the bones to each other holding them together while cartilage reduces friction between bones. The femur and the patella are connected to the tibia and fibula. This consists of muscle that can contract and relax and keeps the skeleton attached to the body. The heart is an involuntary muscle that beats to circulate blood around the body. This type can transmit messages to the brain and from the brain to the rest of the body to warn of pain and for movement.(touching) Part B (iii) Explain the difference between benign and malignant tumours? I will now explain the difference between benign and malignant tumours and their different effects on the body and how they affect us. Tumours are cells that escape from their normal function and multiply out of control. They then form a lump known as a growth or a tumour. (Class notes 2015) Benign A benign tumour is not cancerous and does not spread cancer to the rest of the body it is an isolated growth usually grows in size and can put pressure on the area it’s growing in. The benign tumour can be dangerous if it grows on the brain although it’s not cancerous it can continue to grow in size and put pressure on the brain and lead to major problems. Malignant All malignant tumours are cancerous and can spread through the body causing secondary tumours or metastases. Malignant tumours spread through the blood and lymphatic system around the body. Some malignant tumours can spread very quickly and aggressively to other parts of the body even though the primary tumour may still be small while sometimes they can grow slower and not spread as quickly. Through research it has become clear that malignant brain tumours are the most aggressive and have the ability to spread to the spine and other parts of the body. Although malignant tumours are treatable by surgery to remove the tumour and also may require a follow up treatment of radiotherapy and chemotherapy to rid the body of anymore cancerous cells malignant tumours can return.(nhs.uk 2015) Part C (ii)Explain the structure and function of each element of the urinary system? The function of the urinary system is to clean the body of waste products and excess fluid and to also produce a hormone controlling at the rate red blood cells are made and the enzyme for regulation of blood pressure. Structure Function Kidney The kidney is a bean shaped organ that we have two of containing around 1 million nephrons in each kidney They are around 11cm long and 6cm wide weighing 150g. Kidneys are inside a membrane known as the renal capsule to protect it from trauma and infection. There are two main areas the renal cortex and the renal medulla. The function of the kidney is to clean and filter your blood to also recycle fluids and nutrients required by the body and produce urine for excretion. Ureter The ureter is around 12 inches long and connects kidneys to the bladder it’s made up of muscular tissue that contracts and also contains mucous to help prevent infection. The function of the ureters is to carry the urine from the kidneys down to the bladder. Bladder The human bladder is a hollow sac for storing urine it is comprised of three layers of smooth muscle and also coated with a mucous membrane and located in the pelvic area. The bladders function is to store the urine. When the walls of the bladder contract this results in urination. The normal bladder can hold approx. 470ml of urine. In the bladder we have an internal sphincter which relaxes voluntarily to expel urine. Urethra This is a tube that connects the bladder to the outside of the body. The function of the urethra is to carry the urine from the bladder to the outside of the body it’s longer in men than women. Part C (iii)Draw the structure of a Nephron and explain how it produces urine? Below I will draw the structure of the nephron and explain how the nephron produces urine to rid the body of waste fluids. We do not realise how important it is to dispel urine and how important it is to survive. Each kidney contains about 1 million nephrons these are the filtration system for the body to clean the blood and expel waste and reabsorb nutrients. Filtration in the Bowman’s capsule. Blood travels into the kidneys by the afferent arterioles. These are small blood vessels that turn in to the glomerulus. Meshes of capillaries that are surrounded by the glomerular are also known as the Bowman’s capsule. The blood in these capillaries are under pressure and the capillary walls can let water and other materials through into the capsule. The capsule is a gathering point for the waste products of the blood. Although it has collected other materials that are not waste and shall be absorbed by the nephron as they pass through. (An introductory guide to anatomy and physiology (Louise Tucker) 4th edition) Re-absorption in the convoluted tubule. When the filtered materials are collected by the capsule they move into a system of twisted tubes that are known as convoluted tubules. The tubes that flow away from the bowman’s capsule are the proximal convoluted tubules. These flatten out to form a long loop, called the loop of Henle which flows to the medulla and back to the cortex. Lastly there is more twists called the distal convoluted tubules This is where the reabsorption takes place in these tubules. The cells in the lining can absorb any water, ions and water that the body requires and shouldn’t be disposed of as waste. Only 1% of liquid that travels through the Bowman’s capsule is expelled as urine all the rest is reabsorbed. (An introductory guide to anatomy and physiology (Louise Tucker) 4th edition) Collection in the pelvic calyces This is where the nephron flattens out into a straight collecting tube in the medulla. These tubes form a collection called the pyramids of the medulla the tops of these travel up to the renal pelvis. These branches of the pelvis attach to the tops of the pyramids and gather the waste liquid. It’s the funnelled back towards the pelvis and then empties into the ureter and then can travel to the bladder and down to the urethra to be expelled as urine. (An introductory guide to anatomy and physiology (Louise Tucker) 4th edition). (iv)Name and explain three diseases /disorders which affect the urinary system? Kidney stones These are solid stones which are made up from deposits from substances that are found in urine. They form and are found in the renal pelvis, ureters and the bladder they are very sore on the patient and sometimes require surgery for removal or can be broken down with laser treatment and then passed in the urine. Incontinence This is involuntary defecation or urination but mainly urination that is more common in the elderly, pregnant women or women that have had babies. It is the involuntary leaking of urine and also happens from sneezing or coughing or sometimes the bladder may be full and you may not make it to the toilet in time. It is very embarrassing for the people who suffer with it. Urethritis This is an inflammation of urethra and causes painful urination. This is the tube that carries the urine from the bladder to be expelled by the body. It is a bacterial infection and causes discomfort when urinating it can burn the sufferer when dispelling urine and it may cause discharge also. Though more common in women due to women having a shorter urethra than men. Bibliography (An introductory guide to anatomy and physiology (Louise Tucker) 4th edition) (Class notes 2015) (clevelandclinic.org 2015) (healthline.com 2015) (nhs.uk 2015) (webmd.com 2015)

Effectiveness Of Therapeutic Play Health And Social Care Essay

This chapter dealt with literature mention sing curative drama and painful processs and surveies related to Gate Control Theory. This chapter besides dealt with conceptual model of Melzack and Wall ( 1965 ) Gate Control Theory. CHAPTER-III RESEARCH METHODOLOGY This chapter deals with the methodological analysis selected for measuring the effectivity of curative drama on degree of hurting during endovenous canulation. The function of methodological analysis consists of processs and techniques of carry oning a survey. ( Sharma,1990 ) Methodology is a important portion of the research under which the research worker is able to project a bluish print of the research undertaken RESEARCH APPROACH The subdivision of research attack is the basic process for carry oning a research question. In position of the nature of the job selected and aims to be accomplished, a quantitative appraising research attack was considered appropriate to measure the effectivity of curative drama before making endovenous canulation among kids admitted in selected infirmary, Salem. RESEARCH DESIGN Research design is the overall program for turn toing research worker ‘s inquiries including specification for heightening the survey ‘s unity. ( Polit and Beck, 2004 ) Choice of design is based on the intent of the survey. The research design adopted for the survey was Non-equivalent Post trial merely control group design. Group Day 1 Experimental group X O1 Control group O1 Fig-3.1: Conventional representation of research design Keies: Ten: Intervention on curative drama. O1: Post-test merely to measure the degree of hurting during endovenous canulation. VARIABLES UNDER STUDY: A variable is a mensurable or potentially mensurable constituent of an object or event that may fluctuate in quality or measure from one person, object or event to another single object or event of the same general category. ( Basavanthappa, 1998 ) The variables under the survey was the followers, Independent variable: Harmonizing to Polit and Hungler, ( 1999 ) the independent variable is believed to care or act upon the behavior and thoughts. In this survey the independent variable refers to curative drama before making endovenous canulation. Dependent Variable: The dependant variable is the variable, the research worker is interested in understanding, explicating and predating. ( Polit and Hungler, 1999 ) In this survey the dependant variable refers to the degree of hurting during endovenous canulation among kids. Extraneous Variable: The variables that are present in research environment which may interfere with research findings by moving as unwanted independent variable. ( Woods and Khan, 1994 ) In this survey it refers to selected demographic variables such as age in old ages, gender and behavioral response to endovenous canulation. Setting OF THE STUDY: The scene of the survey is the physical location and status in which information aggregation takes topographic point. ( Polit and Hungler, 2009 ) The survey was conducted in Pranav Hospital, Salem. It is located near to New Bus Stand and about 2kms distance from the establishment, where the research worker is analyzing. The infirmary is 150 stratified multi forte infirmary with 30 bed in Paediatric unit. Population: Harmonizing to Polit and Beck. , ( 2004 ) population is the full collection of instances in which a research worker is interested. Population may be of two types, accessible population and mark population. In this survey two are described. Target Population: It refers to the population that the research worker wishes to do a generalization. In this research the mark population was kids acquiring admitted in Pranav Hospital. Accessible Population: It refers to the sum of instances which confirm to the designed standards and which is accessible to the research worker as the pool of topics or objects. In this survey the population consisted of kids acquiring admitted in Pranav Hospital who were undergoing endovenous canulation during the period of survey. Sample: Harmonizing to Polit and Beck. , ( 2004 ) sampling is the procedure of choosing a part of population to stand for the full population. Sample is the subset of population elements. In this survey the samples selected from kids of age group 3-6 old ages undergoing endovenous canulation in Pranav Hospital who fulfil the inclusion standards. Sampling TECHNIQUE AND SAMPLE SIZE Thomas. , ( 1990 ) defines trying is the procedure of choosing units for survey from a population. In this survey Purposive sampling technique was used to choose sample. Sample size was 20 for control group and 20 for experimental group. CRITERIA FOR SAMPLE SELECTION Inclusion Standards: The topic was selected based on the preset standards, aˆ? Children between the age group of 3-6yrs. aˆ? Children who had admitted in pediatric ward for the intervention through endovenous canulation. Exclusion Standards: aˆ? Mentally challenged kids. aˆ? Children with critically sick or of exigency admittance. DESCRIPTION AND INTERPRETATION OF TOOLS The instrument selected in research must be vechile that obtain best informations for pulling decision to the survey. ( Treece and Treece, 1986 ) The tool developed based on the information gathered from relevant literature reappraisal. The content cogency of the demographic informations and independent variable was established by obtaining sentiment from 5 experts ( 3 Nursing experts and 2 Medical experts ) . Tool -1: Demographic informations: It includes age in old ages, gender, behavioral response to endovenous canulation of the kid. Demographic information of the instrument was non scored but used for descriptive analysis. Tool-2: Wong-Baker Faces Pain Rating Scale: This evaluation graduated table is recommended for kids of ages 3and older. It consists of mark 0,2,4,6,8,10. During the clip of endovenous canulation the facial look of the kid was assessed by the research worker with this hurting graduated table. Face 0-no injury, Face 2-hurts merely a small spot, Face 4-hurts a little more, Face 6-hurts even more, Face 8-hurts a whole batch, Face 10-hurt every bit much as we can conceive of. Based on the hiting the hurting degree was assessed. Development of curative drama for direction of painful processs: A curative drama on direction of painful processs was prepared to diminish the degree of hurting during endovenous canulation. The stuffs used were venflon without stillet, plaster, stuffed doll with endovenous injection site, splint. The presentation was done by the research worker utilizing the needed stuffs and so the kid was allowed to pattern the endovenous canulation for the doll. This method was done before making endovenous canulation to the kid by the staff nurse. VALIDITY OF THE TOOL AND INDEPENDENT VARIABLE: The content was validated by the same 5 experts who validated the tool ( demographic variable ) and independent variable as per the standard. Harmonizing to experts suggestions the necessary images were included in the independent variable. It was translated in Tamil and once more retranslated in English by the linguistic communication experts severally. PILOT STUDY: Pilot survey is the little scale version or a trail tally done in readying for major survey. ( Polit and Hungler, 1999 ) The Pilot survey was conducted in SKS Hospital at Salem. After acquiring the written permission from concerned authorization, researcher conducted survey from 4.08.2010 to 11.08.2010 among kids who underwent endovenous canulation. The intent of the survey was explained to the samples and their parent every bit good as written consent was obtained from them. The entire size was 2 for control group and 2 for experimental group. Purposive sampling technique was used for sample choice. For the control group without giving intercession, the degree of hurting was assessed. A concise information analysis done by utilizing descriptive and illative statistics. The consequence of the survey showed the average station trial degree of hurting for control group as 90 % and for experimental group as 50 % . After that the ‘t ‘ value was calculated to happen out the important difference between the degree of hurting for control group and experimental group at P & lt ; 0.05 degree of significance. No important difference was seen between the control group and experimental group. No association was found between station trial degree of hurting and their age, gender and behavioral response of the kid. Since the sample size was merely 2 for control group and 2 for the experimental group. so the survey was non found as important in ‘t ‘ trial and chi-square trial. DATACOLLECTION PROCEDURE: Harmonizing to Polit and Hungler. , ( 1999 ) , â€Å" Data aggregation is the assemblage of information needed to turn to a research job † . Data aggregation for the survey was done from 18.08.2010 to 05.09.2010 in Pranav Hospital at Salem. Initially the research worker got the permission from the concerned authorization. Then the population were identified from the kids who have got admitted in the infirmary were selected by utilizing purposive sampling technique based on the inclusion standards. The sample size was 20 kids as control group and 20 as experimental group and the intent of the survey was explained to the samples and their parent every bit good as willingness to take part was assured by taking written consent of the parent of each sample and giving confidence for maintaining their information confidentially. At first the control group was selected and no intercession was given. On the twenty-four hours of admittance the hurting degree was assessed, during endovenous canulation by utilizing Wong-Baker Faces Pain Rating Scale. After the control group got finished, the experimental group was selected by purposive sampling technique. On day-1, after the kid got admitted, the parents and the kid were introduced by the research worker. The intercession on curative drama given. A particular room was provided to them in the presence of playthings. The research worker clearly explained and taught about the curative drama by utilizing a stuffed doll and the other needful stuffs. The research worker demonstrated the curative drama. Then the research worker made the kid to pattern the endovenous canulation on the doll by doing an unreal endovenous canulation site utilizing a venflon without stillet. After the intercession the research worker identified that the kid got relaxation from the emphasis of hospitalization. The continuance of the intercession was 20 proceedingss. After the intercession the kid was made to be ready for endovenous canulation. The endovenous canulation was done by the staff nurse. The post-test degree of hurting was assessed during the endovenous canulation by utilizing the same tool. Plan FOR DATA ANALYSIS Collected informations were planned for analysis by utilizing the frequence distribution, descriptive ststistics ( mean, SD, average mark per centum ) and illative statistics ( pupil ‘t ‘ trial, Chi-square trial ) .This was done to happen out the effectivity of curative drama degree of hurting among kids who underwent endovenous canulation. Summary This chapter dealt with the methodological analysis of how the research worker had planned and organized for the informations aggregation, the method of hiting and description of tool, pilot survey, informations aggregation process and program for informations analysis.

What was the most important cause of world war 1 - 1229 Words

What was the most important cause of World War 1 On Sunday 28th June, 1914, the heir to the Austro-Hungarian Throne, Archduke Franz Ferdinand, and his wife, were assassinated by Gavrilo Princip, part of a Serbian Terrorist Group, called the Black Hand at Sarajevo, the capital of Bosnia and Herzegovnia. This event and the tension between Europe was a trigger that set off declarations of war and resulted in World War 1. Tensions had been building up in Europe for decades through Nationalism, Imperialism, Militarism, and Alliances, and these tensions had reach such a point that an event was needed to break these tensions. This was the assassination of the Archduke. Therefore the assassination, Nationalism, Militarism, Imperialism and†¦show more content†¦Furthermore, Britain in particular, used its navy and merchant navy to dominate overseas trade, which also provided a source of irritation to the Germans. 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Isolation of Mitochondria - 2221 Words

Assay of succinate dehydrogenase of after isolation of mitochondria in Cauliflower (Brassica oleracea) using differential centrifugation. Kelly M. Messick, Rebecca Conner Department of Biological Sciences, Salisbury University, Salisbury, MD, 21801 U.S.A Address for correspondence: Kelly M Messick Department of Biological Sciences Salisbury University Salisbury, MD 21801 Phone: 410-546-2060 Fax: 410-543-6433 e-mail: km96536@gulls.salisbury.edu Running title: Assay of succinate dehydrogenase. Introduction Cell fractionation is a very important procedure in cell biology and can be very useful for studying different organelles. By fractionating, we mean separating or dividing the cell into different component parts.†¦show more content†¦A fraction that has high content of the specific organelle and low contamination by other organelles is desired however a fraction with highly purified mitochondria is better even if the most mitochondria is found in other fractions. The purity of mitochondrial fractions is usually determined by enzyme marker detection assay(Hajek et al 2004). In our experiment we will use differential centrifugation to isolate the mitochondria of cauliflower and then assay SDH activity using a fixed time assay. We will then measure protein content in our fractions and calculate specific activity and total activity of our fractions. Materials and Methods Mitochondrial Isolating We used to florets from the cauliflower and disrupted the cell walls using a cold isolation buffer (0.3 M D-mannitol, 0.02 M phosphate buffer, pH 7.2) and an abrasive, we then strained the homogenate with cheesecloth into a centrifuge tube suppoerted by ice. The homogenate was then centrifuged at 600 g for 10 minutes at 4⠁ °C. The postnuclear supernatant was removed and centrifuged at 12,000 g for 30 minutes at 4⠁ °C. The post mitochondrial supernatant was removed and the mitochondrial pellet was resuspended in a cold assay buffer (0.3 M D-mannitol, 0.01 M KCl, 0.005 M MgClâ‚‚, 0.02 M phosphate buffer, pH 7.2). Both samples were stored atShow MoreRelatedSummary Of Mut-OX In A549 Cells1163 Words   |  5 PagesIn this SA, we will study translocation of DNA damage repair proteins from the cytoplasm to mitochondria in response to mtDNA damage induced by mt-OX in A549 cells. We will treat A549 cells with mt-OX as mentioned in SA#...., followed by using a SILAC method as desc ribed above. We will perform differential ultracentrifugation to isolate cellular fractions as previously described (18). The purity of cytoplasmic and mitochondrial fractions will be assessed by western blotting using IÃŽ ºB-ÃŽ ± and Tom20Read MoreEssay On Lysosome869 Words   |  4 Pagesto the autophagosome membrane and become the active form needed during the autophagic process, LC3BII (Pankiv, 2007; Kabeya, 2000). 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The Crucible By Arthur Miller - 1075 Words

The Crucible by Arthur Miller is a drastic play overflowing with stereotyped characters. It takes place in Salem, Massachusetts in the year of 1692. There are suspicions of witchcraft taking place throughout the town. Abigail Williams is the start of it all. If you weren’t actually involved in all the witchcraft hysteria, you were to blame. John Proctor, an innocent Puritan farmer, was one to get involved. He had a fallacious reputation with Abigail Williams. Though Proctor was married, Abigail’s love for him still endured. She wanted him back, so the only way of doing so, she would have to get rid of his wife. Abigail not only got rid of Elizabeth, Proctor’s wife, she got rid of a lot more innocent people as well. They were hung if they†¦show more content†¦At the beginning of the play, his best possession is his name and the integrity associated with it. He didn’t want anyone to figure out he had an affair with Abigail, so he kept quiet. But in the end of the play, he confessed of being an adulterer. His attitude towards the whole thing changed. He cared deeply about his reputation and what people thought of him. Towards the end, he didn’t care. He wanted his wife out of jail, and put Abigail in. Proctor is not only motivated by his wife, he is motivated by honor. He wanted to have others respect him, but he deserved respect for himself. He demonstrated his notion of self-worth and sacrifice. Proctor stands for the truth and resists the social paranoia of the town. He is able to stand for his word and not sign a false accusation. He acted upon what he believed. Proctor is irritated with the whole witchcraft fiasco. He wants to put it to an end, along with Abigail. While Proctor was being accused of witchcraft by the judges, he decided to confess. He didn’t confess to being involved in the witchcraft, but he confessed about his adultery with Abigail Williams. He wanted to prove to the court that she had a moti ve for wanting to accuse Elizabeth Proctor of witchcraft. He wanted to make it evident to the court that Abigail’s desire to do away with his wife is what has driven her to accuse Elizabeth as well as the others. Proctor is in hope for discrediting Abigail in the eyes of the court. Thinking

My Windsor investigation Free Essays

string(82) " also visited the crooked house near the extremely small Queens Charlotte Street\." Tourism is the business of going on holiday and making services at the same time. This is more people being provided with jobs. There has been an increase in tourism, because people have more money nowadays to spend on holidays. We will write a custom essay sample on My Windsor investigation or any similar topic only for you Order Now There are many reasons for this. Some are paid holidays, cheaper flights, and more leisure time. All of these factors make it possible for a person to be able to go on holiday. Also, some parts of the government occasionally promote tourist areas which they support in making successful. There are quite a variety of tourist destinations. There are adventure holidays, such as Disney Land, beach holidays, such as Spain, which is most commonly associated with sunny weather and attractive beaches. Many also like to go on ski resorts, such as Switzerland and historical sites are as popular type of tourist destination. Windsor is a historical destination, because of its famous Windsor Castle. Tourism is important to Britain, as, creates employment opportunities, in turn improving Britain’s economic status. Tourism in popular places, such as Windsor builds a better reputation for Britain across other countries. In addition, the money tourists spend helps Britain economically. Tourism provides potential for future visits and business, and is a good way of creating cultural exchange, and allowing others to learn about the country and traditions within. Tourism offers the opportunity to diversify local economy and helps the conservation of famous sites around many different countries. A good tourist centre requires mainly exciting attractions. Most tourists would want tour guides to take them around places. Transport would be essential for being able to move from place to place properly. Most tourists like to shop for souvenirs so shops would be seen as something important for tourists if they wanted a reminder of their visit. Good accommodation such as hotels and places to eat, e.g. a fast food restaurant is what tourists would also need. Friendly people and a good, safe environment would also be something required by many tourists. A good place to live would require, first of all, houses to live in, good, clean, quiet environment. Families would want satisfactory schools for their children. Residents would want local superstores or shops, to buy food and luxuries etc. Hospitals and any other sort of medical aid is an essential for every resident living in a certain area, e.g. doctors, dentists. People would want places of entertainment to attend. These can be cinemas, pubs, and clubs or anywhere where a person may want to socialise. A resident would need some extent of safety and security to ensure that they are living in a safe environment in case anything should happen, so police would have to be in hand to help secure the area. Local job vacancies would be probably more convenient than essential for residents, and would be preferred by people living in Windsor. Car parking availability should be provided as well. Tourism affects Windsor in many ways; both in negative and positive ways. It can cause crowded streets, inflated prices, and traffic, parking difficulties, litter and pollution. Particular activities that tourists take part in contribute towards environmental damage. Purchasing of goods by tourists in Windsor cause inflated prices, which makes it difficult for most residents in Windsor to afford many of the things sold in stores. Litter and pollution make it increasingly difficult in Windsor to maintain cleanliness in the environment. Parking difficulties can cause conflict between residents and tourists, and make it tricky for either residents or tourists (or both) to actually travel anywhere or find a decent parking space which is convenient for them. However, on the other hang, tourism in Windsor encourages employment, raises status for Britain, improves facilities and it also helps to preserve historic sites such as its famous Windsor Castle. Tourism also brings more income to the country enhancing the state’s economic wealth. Windsor Windsor is on the west side of London, near Heathrow airport, in Berkshire. The attractions that Windsor has got are the Windsor Castle, Guildhall (where Prince Charles and Camilla Bowles got married), Lego land, and Curfew Tower. People visit these attractions, mainly for its historic content. Whereas many others visit it for the adventure of Lego Land. METHOD At the beginning of the project, our class had to learn about Windsor. We learnt many basic facts about Windsor, and the fact that it is a tourist destination. We did class work, engaged in discussions about Windsor, and had to research facts about Windsor as homework, as part of our own independent research. We also learnt about tourism itself, and its effects. The sources we used to gain this information were secondary sources. This was the internet, textbooks, the library etc. We then drew up an introduction to our project based on our knowledge about Windsor and tourism. Once learning about Windsor and tourism, we then practised the questions on each other in class in order to prepare us for the real thing. After using secondary research, we then moved on to using primary research. This was carrying out our surveys in Windsor. We went to Windsor on 3rd May. We travelled by coach for roughly one hour, and we arrived at a coach park. One of the first things we, as a group noticed was the large amount of other coaches that were there and lots of tourists that were coming out of the coaches. The first thing we did was had a tour around the area. There were signposts displaying greetings in many different languages. Some of which were French, German, and Japanese. We passed many tourist shops and many high street shops, which also exist in London, and were obviously aimed at the residents. We then took a visit to the River Thames, which was a lot narrower in Windsor, than in London and we saw many swans. We visited Peascod Street and the Guildhall. We also visited the crooked house near the extremely small Queens Charlotte Street. You read "My Windsor investigation" in category "Papers" Nearby, was Nell Gwynne’s House, where Shakespeare wrote one of his famous plays â€Å"Merry Wives of Windsor†. We witnessed the changing of the guards, and visited the outside of the castle. It was very big, with guards standing at the entrance, with lots of tourists coming in and out, mostly looking at their maps for directions. We also saw the â€Å"long walk†. We then all gathered around the castle, from where we were distributed into small groups of mostly four. We had to go around asking our questions to the people who looked like residents and people who looked liked tourists. We had to do this to five of each. This was easy for me and my group. We approached the people in a polite way and asked the questions in the survey thoroughly. The residents were very polite and enthusiastic. However, although it was very easy to interview the residents, the tourists were more difficult to obtain our results from. The reason we felt for this was because many of them were foreign and had difficulties speaking English. However, in the end, we received our results, and our (me and my group’s) research was successful. This was because we managed to obtain a balanced sample of people to interview. We tried to make sure we interviewed people of different ages and genders. This is important, as a balanced opinion is needed to give fair results, and a better insight into the people’s views about the topic. When we finished our surveys, we were allowed to have some free time to eat and rest. We then met up at an agreed time back outside the castle, from where we took off and left to come back to school. MY RESULTS This graph shows us how the tourists rated the facilities in Windsor. This shows me that the residents think that the best things in Windsor are the signposting, the historical interest, the cleanliness and the friendliness. The fact that the tourists find the residents friendly possibly shows that the residents do not mind the tourists being there and that there isn’t a conflict of interest between tourists and residents in Windsor. The things that the residents rated the lowest were the value for money and the amenities. This means that the prices are expensive in Windsor and the tourists see this as a problem. This graph tells me what the tourists I surveyed thought that needed improvement in Windsor. This graph tells me that the tourists think that the shops and pedestrian areas need least improvement in Windsor, but parking and availability of toilets is limited and a problem for the tourists in Windsor. This shows that there could be a conflict of interest between the tourists and residents, as it appears to be a battle of who can get parking spaces etc. This graph shows us what attractions the tourists prefer to go to. This graph tells us that most of the tourists do not like Lego Land as an attraction to visit in Windsor nor do they like the tours around the area. I think this is, because all of the tourists surveyed were adults and were without children so were not expected to go to Lego Land. The most popular tourist attractions in Windsor are the river and the shops. The shops in Windsor were very good, as they had all of the things a tourist would look for, e.g. souvenirs. This pie chart shows me how many tourists were willing to visit Windsor again. This graph shows me that 16 (80%) out of 20 tourists are willing to visit Windsor again, and 4 (20%) aren’t. This statistic could be used to conclude that Windsor is in fact a successful and satisfying tourist centre and that there isn’t a conflict of interest between the tourists and the residents in Windsor. This graph shows me if the tourists were willing to recommend visiting Windsor to their friends or family. 19/20 said yes, they would. That is 95%. Only one person said they wouldn’t recommend Windsor to friends and family. This shows me that the tourists enjoyed their visit to Windsor. + This graph shows us what problems the tourists cause in Windsor. From my results, I can judge that the biggest problems tourists in Windsor cause are parking difficulties and crowded streets. This could be a cause for conflict between the tourists and residents. The least problems caused by tourists, according to my graph, shows inflated prices and too many shops aimed at tourists. These results are surprising, as a successful tourist centre like Windsor would be expected to have high prices, which would affect residents living in the area. This graph illustrates whether the residents avoid shopping in certain areas because of the tourists. I can see from my results that most of the residents do not avoid shopping in any parts of the town, because of the tourists. This could be because the tourists and residents go to different kinds of shops, i.e. tourists would be expected to go to shops which sell things for tourists, e.g. souvenirs etc., whereas residents are more likely to go to a superstore. The other argument could be that the residents and tourists get along well with each other, and have no problem with going to the same shops, which would prove that there is no conflict of interest. This graph shows us what benefits the tourists bring to Windsor. This graph tells me that the biggest benefits that tourists in Windsor bring to the town are employment, income, and preservation of historic sites. All of these things show the most obvious benefits which tourism would bring to an area. The least benefits they bring to the town are status. This result is particularly surprising, as I expected people to say Windsor’s status has been affected by tourism, especially as Windsor is popular as a tourist destination, and this is widely known. This graph shows us how the residents rate the facilities in Windsor. This graph shows me that the residents think that the historical interest, the pedestrian areas, and the food in cafes and restaurants are the best things in Windsor. The tourists in Windsor agreed that the historical interest in Windsor is good as well as the food. However, they think that parking and amenities aren’t very good in Windsor. I think that this is because of the tourists being in Windsor. Parking availability would probably be a lot better if there were no tourists visiting Windsor, as well as amenities provided there. This graph shows us what the residents think would improve Windsor. This graph shows me that the residents would want the biggest changes made to supermarkets, toilets and parking spaces. The toilets and parking spaces could be a cause of the tourists and the residents wanting to improve these things could mean that they have a problem or problems with the tourists’ visits. The things that the residents think need the least improvement are the shops aimed at tourists, the amount of tours, and the opening times of the castle. This graph shows us who the residents think the council favours more. From these results, I can see that the changes introduced by the council have mostly got the balance right between the tourists and residents. This means that Windsor is not only good as a tourist centre, but as a residential area too, which means that the residents in Windsor are satisfied with where they live. This is true, as there were no residents who thought the council favoured the tourists more, but a minority said the residents were favoured more. This graph tells us if there is a conflict of interest between the tourists and residents. Most of my residents said ‘no’. This shows me that generally, the residents do not think there is a conflict of interest between the residents and the tourists. RESULTS Tourists Results: There were many streets/areas which seemed to be aimed at the tourists. Some of these were: 1 Thames Street 2 Station Parade 3 Castle Hill 4 Eton All of which have particular things to attract the tourists, which stands out from the rest of the areas in Windsor. Most of the tourist sample came from the UK, with 11/20, however a similar amount of tourists came from abroad with 9/11. This shows no correlation between Windsor and the tourists who visit it. Most of the tourists i surveyed travelled to Windsor by car, which were 8/20. This shows me possibly that most of the tourists were independent visitors. The least used transport to Windsor by the tourists was a bus. The most popular of attractions for the tourists was the river, the Guildhall, and the shops. 50% of the tourists said they would be visiting the river, and 30% of the tourists said they would be visiting the Guildhall, which is still quite a low score. But, with the highest score, 60% of the tourists said that they would be shopping in Windsor on that day. The tourists rated all of the facilities in Windsor over four, with the exception of only a few. These were: 1 Value for money 2 Amenities (such as toilets) 3 And information on things to do. These however, still scored a rating of over three, which is still a high score, which shows that Windsor is good as a tourist centre, as it provides all of the needs for tourists. The most popular facilities were: 1 Signposting 2 historical interest 3 cleanliness 4 and friendliness These are all very important elements needed for a site to be particularly successful for tourism. Signposting is needed for guidance around the area, and since they were written in many different languages, this is extremely helpful for foreigners. A clean environment is needed for not only a tourist, but residents too. Tourists want a place to feel safe from insanitary when they are visiting somewhere to enjoy themselves. Friendliness also is a very important factor in being successful as a tourist site. Tourists want to feel welcome, and wanted there. If there wasn’t any friendliness in Windsor, the tourists would probably be put off, and not want to visit Windsor again. 80% of the tourists said they would visit Windsor again, and 20% said they wouldn’t, which shows Windsor is a big preference for the tourists, and they were impressed by the visit. 95% of the tourists said they would recommend visiting Windsor to a friend or member of family. Residents Results: There were many streets/areas which seemed to be aimed at the residents. Some of them are: 1. Peascod Street 2. Kind Edward’s Court 3. High Street/Thames Street 4. Around the station From my residents’ sample, I found out that every one that i surveyed lived in Windsor for at least 6 months. A few which I interviewed had been living in Windsor for: 19 years, 28 years, 50 years, 65 years, 40 years, 66 years, etc. So a lot of the residents i surveyed had been living in Windsor most of their lives. All of my residents said they visited the town centre at least once every two weeks. The majority visited the town centre daily, which shows that Windsor is a good place to live in; otherwise my residents wouldn’t have said this. The results from my residents chart shows me that on average, the residents think tourists do not Bring that many problems to the town. However, the problems they did bring were: * Crowded streets * And parking These could cause a conflict of interest between the tourists and residents, as this would mean residents/tourists cannot go to certain places, as they have to worry about parking their car, or be worried about crowded areas. The least problems they caused were: * Inflated prices * Traffic * And shops aimed at tourists. Although the tourists think that there is a problem with crowds, they do not think there is much traffic to the extent of being a problem. This can be expected, as the tourists spend their time walking around more in groups, than using means of transport. The residents all rated the facilities in Windsor over 2.5, which is slightly less that what the tourists rated the facilities. This (although vague) shows that the tourists prefer Windsor to the residents. The lowest scored facilities were the parking and amenities. The tourists share this same problem, as they rated amenities as one of their lowest. This suggests that the council in Windsor should put this as one of their main priorities for improving Windsor for the residents and tourists. The highest rated facilities by the residents were: historical interest, pedestrian areas, and cafes/restaurants. The tourists also rated the historical content of Windsor highly as well. The biggest changes residents thought would improve Windsor for the better were: * Toilets (80%) * Supermarkets (70%) * And parking (85%) The tourists share the same view about toilets and parking. However, compared to the residents, a lot less suggested improvement for these things. 25% of my tourists suggested improvement to toilets and 25% of tourists suggested improvement to parking. The least changes suggested by the residents were for: * Shops (70%) * Amount of tours (70%) * Opening times of the castle (70%) The tourists also agreed that shops didn’t need improvement, (90% said this). Overall, tourists appear to be more content with Windsor than the residents. The most changes needed are for parking and amenities (toilets). This means that the council needs to take this into consideration in order to improve Windsor for tourists and residents. EVALUATION OF RESULTS I can conclude, from everything I have found out, using my graphs/results that Windsor is a successful tourist centre. I have collected sufficient information in backing up this point, which gives me full confidence in stating this. In asking my tourists to rate particular things in Windsor, all were rated over 3 out of 5 for a score, and 8/14 being scored 4 or over. This showed me that as a whole, tourists are satisfied with the content of Windsor and the different services it provides as a tourist destination. Most of the tourists didn’t feel that Windsor needed any improvement, and 80% of my tourists agreed that they would be coming to visit Windsor again in the future. There is some evidence according to my results to suggest that there is a conflict of interest between the tourists and residents in Windsor. A lot of the residents i surveyed claimed they had problems with the crowds and lack of parking availability which was caused by the tourists. This is/would be seen by the residents as inconvenient to a large extent i.e. Windsor is their home and having to live there whilst having no parking space for their car would be very hard to deal with, especially when having to go to work or travel for other needs essential to the residents. Another thing a lot of the residents I interviewed were displeased with was the lack of amenities served in the area such as toilets. This could be because of tourists constantly using them during their visit to Windsor, which a resident could find to be annoying especially if they are out all day, and cannot go home. However besides these facts, I found the residents to be overall fine with the tourists’ visits to the popular town, and felt that there was a friendly atmosphere between the residents and tourists. A considerable amount of residents said that there were no places in the town they avoided shopping in because of the tourists. Also, most of my residents agreed that tourists did bring employment, income and were the cause of conservation of historic locations such as the Castle. The residents rated most of the facilities in Windsor over half, which shows approval of Windsor, including tourism. 16/18 of the residents believed that the council favoured both the residents and the tourists, making sure the balance was right and that neither was losing out. Finally, from the results I have assembled, I can see that the majority of residents I surveyed throughout my trip to Windsor said that there was no conflict of interest between the residents and tourists. The council, however has managed to try resolve the little bit of conflict between the tourists and residents by making sure to fulfil the residents’ needs, by providing them with advantages as citizens in Windsor. One way they have successfully managed to do this, is by providing the residents in Windsor with Advantage Cards. These cards allow residents in Windsor to attend attractions such as The Castle with discounts. Special offers are also given on tour guides around the area’s best attractions. Supplementary litter bins have been made for enhanced hygiene for the residents. Finally, further parking spaces have been set up to the residents’ advantages to ensure no parking difficulties occur, which don’t allow residents to park their cars whilst the tourists are visiting. EVALUATION OF METHOD On our visit to Windsor, the day went generally well. Firstly, the atmosphere was very good. There was a huge mixture of people from different backgrounds and areas, so it was very multicultural. It was a clean, tidy area, with hardly any litter lying around, which showed it was well maintained. I liked the attractions in the area, the shops, and just generally the things in the area. It was a good looking place, mostly because of its cleanliness, and quiet streets. The good weather added to the scenery. The interviews went considerably well. The people in the area were very polite, and welcoming. Most of the people cooperated, in helping me and my group fill in our survey. They seemed very enthusiastic. I was able to get an adequate amount of residents and tourists to fill out my survey, which obviously helped me a lot for my coursework, in creating my graphs and evaluating my results. All of the people I interviewed appeared to understand the meaning and the context of the questions which I asked them, and easily answered them, which was convenient and time-saving for my group. On our day to Windsor, I encountered minor problems. One of these was when I was looking for tourists to interview for my survey. A lot of the tourists at first kept refusing to do the interviews when requested. Some couldn’t speak English which appeared to be the reason. It took a long time to find five tourists who were willing to cooperate, but eventually we did, however it was really time-consuming. I had a few problems when doing my Windsor coursework. One problem was being able to follow up on work inside and outside of school. I also had problems with attendance, which caused me to fall behind slightly at first with my coursework. Occasional difficulties with understanding parts of the project drew me back slightly, but help from my teacher and peers helped me overcome these drawbacks. I managed to get a balanced sample of people to interview. I tried to obtain fair results by interviewing as many men as I could women, and tried to interview people from different age categories. I managed to do this, by referring to my survey and seeing who I had to interview. It is important to have a balanced sample, so that the results I receive are accurate and reliable. CONCLUSION From the start of my Windsor project, my aim has been to successfully answer three questions about Windsor: 1. Is Windsor a successful tourist centre? 2. Is there a conflict of interest between residents and tourists? 3. What has been done to try to solve any conflict of interest In trying to accomplish these questions, I can finally conclude that from all of the information I collected in the duration of this project I have found Windsor to be a very successful tourist centre. The tourists I surveyed agreed that among many other positives in Windsor, the popular destination has good historical content and attractions and is full of friendly people. The tourists were willing to visit the town again in the future and even recommend it to friends and family. Windsor provides the tourists with wide varieties of ways to enjoy themselves and makes sure they have a good visit. The attractions such as Eton, the Guildhall and others play a big part in this as well. I have concluded in confidence as well that there is generally no conflict of interest between the residents and the tourists. The residents seemed happy to be accompanied by the tourists and this was shown through the survey I carried out, where the residents thought the tourists brought benefits to the town such as employment, status, preservation, income. My residents also said there was no conflict of interest between the residents and tourists in Windsor. Any minor conflicts in Windsor between the tourists and residents have been dealt with by the council. The council has introduced Advantage Cards which give residents in Windsor special advantages to visiting attractions, and allowing them discounts and offers on tours. Additional litter bins and parking spaces were also produced so that the residents and tourists have an equal balance in being provided with needs and wants. How to cite My Windsor investigation, Papers

Facing Racism Essay Example For Students

Facing Racism Essay Teenage WastelandParent/Child relationships are very hard to establish among individuals. This particular relationship is very important for the child from birth because it helps the child to be able to understand moral and values of life that should be taught by the parent(s). In the short story Teenage Wasteland, Daisy (mother) fails to provide the proper love and care that should be given to her children. Daisy is an unfit parent that allows herself to manipulated by lacking self confidence, communication, and patience. Daisy lacks self confidence which made it harder to raise her fifteen year-old son Donny. There were many instances where Daisy pondered on what she can do better to help Donny in school, but as she put forth an effort, she always resisted. She remembered when Amanda was born. Donny had acted lost and bewildered. Daisy had been alert to that of course, but still, a new baby keeps you busy of course.(570) When Daisy saw this happening, she never stopped to reassure Donny that even though he had a sister, it was not going to change their relationship. Daisy should have reassured her son by correcting the problem as soon as it surfaced, then Donny should have understood. When Donny started to have problems in school, Daisy gave up without trying, and let a tutor dictate her sons activities especially when the teacher questions Daisy about Donnys actions, Daisy replied, Oh Im sorry, Miss Evans, but Donnys tutor handles these things now (572) In school Donnys behavior changed soo drastica lly that he started to stay out late and Daisy just sat back and let this happen. The tutor had sat down so many rules!She were not allowed any questions at all about school, nor were to speak with his teachers,Only one teacher disobeyed(572) Because Daisy didnt believe in herself or her word, she let others control and therefore his behavior worsened. Communication is the number one aspect of relationships because it is what allows the parent /child to become more familiar with each ones thoughts and feelings. But he tells us he doesnt have any homework or he did it all in study hall. How are we supposed to know what to believe?(569) This example shows that Donny and Daisy are very distant. Daisy should have looked over his homework to show consideration as a parent to correct and make sure his work is completed, but instead she put too much trust in him that he took advantage of her. Patience is needed to help build a positive relationship with one another in order to get better results. Even though Daisy is raising more than one child, both of them require the same amount of attention. Youll never guess what happened at., Amanda would begin, a Daisy would have to tell her, Not now honey.(569) Daisy seemed as though she set high expectations for her children and gave up easily as she attempted to help him with his homework. She sat next to him as he worked, trying to be encouraging, sagging inwardly as she saw the poor quality of everything he did-the sloppy mistakes in math, the illogical leaps in English(569) If Donny noticed that his mother had patience with him while he did his school work, then maybe Donny would not hesitate or feel intimidated to seek help from his mother. Parent/Child relationships are very important for the child to have in their life. In this story, the author portrays many examples on what kind of a parent Daisy is. Many of these examples can be related to relationships today to show that many parent lack something that separates them from their loved ones that breaks the communication process. These relationships should be consist and positive in order for the child to grow with values that help them in the future so they can play an important role in their life.